cad file for the microfluidic chip design  (Mendeley Ltd)

Journal: STAR Protocols

Article Title: Microfluidics for single-cell lineage tracking over time to characterize transmission of phenotypes in Saccharomyces cerevisiae

doi: 10.1016/j.xpro.2020.100228

Figure Lengend Snippet: Cell-tracking microfluidics chip (A) Cell- and lineage-tracking custom microfluidics design (figure modified from Figure 1A in Bheda et al., 2020a ). The chip is designed with 16 independent microchambers, with each having its own media and cell inlet and outlet channels (represented by different colors), where different strains or conditions can be tested simultaneously. Each microchamber has 8 microchannels for trapping the yeast such that 8 regions containing cells of interest can be imaged per strain/condition ( Goulev et al., 2017 ). (B) Mold fabrication using photomasks and SU-8 photoresist. Photomasks are made from CAD files designed for each layer of the microfluidics chip, then printed onto slides. The mold is made by 2-layer photolithography using a silicon wafer. The process for each layer involves using a spin coater to evenly spread SU-8 photoresist on the wafer and UV treatment through each photomask to transfer the design onto the wafer. This process results in a negative replica mold that can be used repeatedly to prepare PDMS microfluidics chips. (C) Preparation of a PDMS chip stepwise from left to right. Liquid PDMS mix is poured into the replica mold and baked. The solidified PDMS is then assembled into a microfluidics chip by punching holes, treating with O 2 plasma, and attaching to a coverslip. For details see text.

Article Snippet: The CAD file for the microfluidic chip design is available on Mendeley Data ( ).

Techniques: Cell Tracking Assay, Modification, Clinical Proteomics

Journal: STAR Protocols

Article Title: Microfluidics for single-cell lineage tracking over time to characterize transmission of phenotypes in Saccharomyces cerevisiae

doi: 10.1016/j.xpro.2020.100228

Figure Lengend Snippet: Cell-tracking microfluidics chip (A) Cell- and lineage-tracking custom microfluidics design (figure modified from Figure 1A in Bheda et al., 2020a ). The chip is designed with 16 independent microchambers, with each having its own media and cell inlet and outlet channels (represented by different colors), where different strains or conditions can be tested simultaneously. Each microchamber has 8 microchannels for trapping the yeast such that 8 regions containing cells of interest can be imaged per strain/condition ( Goulev et al., 2017 ). (B) Mold fabrication using photomasks and SU-8 photoresist. Photomasks are made from CAD files designed for each layer of the microfluidics chip, then printed onto slides. The mold is made by 2-layer photolithography using a silicon wafer. The process for each layer involves using a spin coater to evenly spread SU-8 photoresist on the wafer and UV treatment through each photomask to transfer the design onto the wafer. This process results in a negative replica mold that can be used repeatedly to prepare PDMS microfluidics chips. (C) Preparation of a PDMS chip stepwise from left to right. Liquid PDMS mix is poured into the replica mold and baked. The solidified PDMS is then assembled into a microfluidics chip by punching holes, treating with O 2 plasma, and attaching to a coverslip. For details see text.

Article Snippet: The CAD file for the microfluidics chip design used in is available at Mendeley Data ( https://data.mendeley.com/datasets/yr2nrysyyx/1 ) ( ).

Techniques: Cell Tracking Assay, Modification, Clinical Proteomics